The blue light-induced interaction of cryptochrome 1 with COP1 requires SPA proteins during Arabidopsis light signaling
Fig 3
Co-expression of SPA1 increases the interaction between COP1 and CRY1 in yeast three-hybrid experiments.
(A) Yeast two-hybrid assay analyzing the interaction between CRY1 and COP1. (B) Yeast three-hybrid assay analyzing the CRY1-COP1 interaction in the presence or absence of co-expressed SPA1. (C) Yeast three-hybrid assay analyzing the CRY1-SPA1 interaction in the presence or absence of co-expressed COP1. Co-transformed yeast cells were grown in darkness for 24 h and exposed to B (50 μmol m-2 s-1) or kept in darkness for 24 h before measuring ß-galactosidase activity. Error bars represent the SEM of three biological replicates. Asterisks indicate significant differences between the indicated comparisons (** p < 0.01, *** p < 0.001, n.s. not significant at p = 0.05).