Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development
Fig 5
Activation of SHH attenuates the increase of tip area and FGF signaling in Ext1 mutants.
(A-H) Representative images of explant cultures treated with DMSO or SAG for 48h. Treatment of Ext1f/f; Shhcre mutant lungs with SHH signaling agonist SAG rescued the tip dilation phenotype. The epithelium was outlined with grey line (E-H). (I -L) The corresponding enlarged view of the boxed area in (E-H). (M) Quantification of average tip area of the buds. Data were presented as mean ± SEM. *p<0.05, n = 3 for each group.(N-Q) Representative images of ISH showing Fgf10 expression of lung explant cultures treated with DMSO or SAG for 48h. Addition of SAG attenuated Fgf10 expression in Ext1f/f; Shhcre mutant lungs. (R-U) Representative images of pERK staining of lung explant cultures treated with DMSO or SAG for 48h, SAG treatment attenuated epithelial pERK staining in Ext1f/f; Shhcre mutant lungs, the epithelium was highlighted by white dashed lines. Scale bars: A-H, 500μm; N-Q,500μm; R-U, 50μm.