A Hox complex activates and potentiates the Epidermal Growth Factor signaling pathway to specify Drosophila oenocytes
Fig 2
PntP1 can induce larval oenocyte fate in the embryonic dorsal ectoderm.
Fig 2A-2C: Lateral views of control PrdG4 (A), PrdG4;UAS-PntP1 (B) and PrdG4;UAS-PntP2 (C) embryos immunostained for the HNF4 oenocyte marker [42]. The PrdG4+ abdominal segments of each embryo are labeled, and the PrdG4+ second thoracic segment (T2) is labeled in the PrdG4;UAS-PntP1 embryo (C). Fig 2D: Lateral view of PrdG4;UAS-PntP1 Drosophila embryo (stage 11) immunostained for Salm. The PrdG4+ thoracic (T2) and abdominal segments (A1/A3/A5/A7) are labeled and reveal increased Salm levels in the dorsal ectoderm. Fig 2E and 2F: Lateral views of AtoG4;UAS-PntP1 (E) and SpaltG4;UAS-PntP1 (F) embryos immunostained for the HNF4 oenocyte marker [42]. The PrdG4+ first abdominal segment (A1) of each embryo is labeled. Fig 2G and 2H: Lateral views of Stage 15 PrdG4;UAS-EGFRDN (E) and PrdG4;UAS- EGFRDN;UAS-PntP1 (F) embryos immunostained for HNF4 reveals the expression of PntP1 in absence of EGF signaling is unable to induce oenocyte formation. The PrdG4+ abdominal segments of each embryo are labeled.