A new role for Rrm3 in repair of replication-born DNA breakage by sister chromatid recombination
Fig 1
Genetic analyses of spontaneous and HO-induced recombination.
(A) Analysis of Leu+ recombination events with the TINV plasmid recombination system, which measures recombination between the two inverted leu2Δ5’ and leu2-HOr repeats of the pTINV plasmid (B) Analysis of Leu+ recombination events with the plasmid-chromosome recombination system that measures recombination between the pCM189-L2HOr plasmid and the chromosomal leu2-k allele (C) Analysis of Leu+ recombination events with the chromosomal system that measures recombination between the leu2Δ5’ and leu2-HOr repeats located on either arm of chromosome III. All experiments were performed in wild-type and rrm3Δ cells either after HO activation in 2% galactose (+HO) or without HO activation to measure spontaneous events (-HO). Diagrams of systems are shown above each graph. Values plotted for each genotype are the average and SD of the median values of three fluctuation tests (each based on 6 samples) performed with three independent transformants in the case of the TINV and plasmid-chromosome recombination systems and the average and SD of 6 independent colonies in the chromosomal system.