Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness
(A) Growth analysis of chimeric Swi4 TFs integrated into the genome in the background of mbp1Δ strain. The strains containing chimeric KlMbp1BD-Swi4 AD (green line) and CaMbp1BD-Swi4AD (light blue line) display similar growth as the mbp1Δ strain (grey line). Strains with chimeric YlResBD-Swi4AD (pink line), NcResBD-Swi4AD (red line) and SpRES2BD-Swi4AD (yellow line) exhibit impaired growth rates relative to the mbp1Δ strain. (B) Quantification of the cell morphology of the different strains by light microscopy manual analysis. Each strain was analyzed in triplicates and at least 100 cells were analyzed and manually categorized (C) Strains morphology was analyzed by microscope. KlMbp1BD-Swi4 AD, KlSwi4 BD-Swi4AD and CaMbp1BD-Swi4AD strains exhibit similar morphology as the mbp1Δ strain, while NcResBD-Swi4AD and SpRes2BD-Swi4AD cells are elongated and exhibit severe defects in cell division.