Gene duplication and co-evolution of G1/S transcription factor specificity in fungi are essential for optimizing cell fitness
(A) Schematic representation of the chimeric Mbp1 TFs based on the substitution of the native Sc Mbp1 DBD by different orthologues. Orthologues from the different clades were chosen based on the fungi phylogenetic tree (Fig 1). (B) In vivo phenotypic analysis of the different chimeric Mbp1s in swi4Δmbp1Δ strain. Substitution of ScMbp1BD by CaMbp1BD, DhMbp1BD, YlResBD, NcResBD, SpRes1BD and SpRes2BD results in non-viability of the cells, while KwMbp1BD and KlMbp1BD exhibit complementation ability. (C) Schematic representation of the chimeric Swi4 TFs based on the substitution of the native ScSwi4BD by different orthologues from the different clades based on the fungal phylogenetic tree. (D) In vivo phenotypic analysis of the different chimeric Swi4 TFs in swi4Δmbp1Δ strain. Substitution of ScSwi4BD by all orthologues across the phylogenetic tree exhibits complementation that supported yeast viability.