Meioc maintains an extended meiotic prophase I in mice
Fig 5
Expression of cyclin A2 and cyclin B3 in Meioc-deficient adult testis and ovary.
(A) Immunofluorescence staining for CCNA2 and SYCP3 in wild-type and Meioc -/- P15 testis. In wild-type, CCNA2 is expressed in mitotic spermatogonia (arrowhead), and is not expressed in cells past the leptotene stage of meiosis (SYCP3+ cells at zygotene stage of meiosis). In Meioc -/- testes, CCNA2 is misexpressed in SYCP3+ cells at zygotene-like stage. (B) Single molecule FISH staining for Ccna1 and Ccna2 in wild-type and Meioc -/- P15 testis. Low magnification image: DAPI staining of germ cells in testis tubule. High magnification images: DAPI is in blue, and single cells are outlined. Single transcripts are detected as individual red or green dots; diffuse staining is background. Ccna2 (green dots) is detected in both wild-type and Meioc -/- testes in mitotic spermatogonia, identified by their position at base of tubule and by their nuclear morphology (wild-type and Meioc -/-, i). Ccna2 is additionally detected in Meioc -/- germ cells that are in middle of lumen (likely meiotic, or else metaphasic cells, ii). Ccna1 (red dots) is detected in late pachytene germ cells in wild-type testis, but not in Meioc -/- germ cells (wild-type and Meioc -/-, ii). (C) Single molecule FISH staining for Ccnb3 and Dazl in late zygotene/early pachytene germ cells from wild-type and Meioc -/- P15 testis and E16.5 ovary. Ccnb3 and Dazl are detected in both wild-type and Meioc -/- germ cells. Scale bar = 10 μm.