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Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors

Fig 2

Acute AMPAR activation triggers stronger elevation of spontaneous neuronal activity in WT than Nedd4-2andi cortical neuron cultures.

(A) Representative raster plots and quantification of spontaneous spikes from 1-min recording (right) of (A1) WT or (A2) Nedd4-2andi cortical neuron cultures before and after vehicle (ddH2O) or AMPA (1 μM) treatment for 15 min. (Vehicle: n = 6 and 9 for WT and Nedd4-2andi; AMPA: n = 7 and 11 for WT and Nedd4-2andi) (B) Representative average traces of 1-min recording and quantification of average spontaneous spike amplitude of (B1) WT or (B2) Nedd4-2andi cortical neuron cultures before and after vehicle (ddH2O) or AMPA (1 μM) treatment for 15 min (Vehicle: n = 6 and 9 for WT and Nedd4-2andi; AMPA: n = 7 and 11 for WT and Nedd4-2andi). The black lines represent the average of all the spikes within representative 1-min recordings. Data are analyzed by a 2-way ANOVA with post-hoc Tukey test and represented as mean ± SEM. The comparison between treatments or genotypes is described with *p<0.05, ns: non-significant. Significant interaction between treatment and genotype was detected in spontaneous spike rate (A3; p<0.05) but not amplitude (B3; p>0.05).

Fig 2

doi: https://doi.org/10.1371/journal.pgen.1006634.g002