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Epilepsy-associated gene Nedd4-2 mediates neuronal activity and seizure susceptibility through AMPA receptors

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Nedd4-2 mediates spontaneous neuronal and synaptic activity.

(A1) A diagram showing two alternative protein products (isoforms 1 and 2) from the Nedd4-2 gene, and (A2) western blot results of Nedd4-2 and Gapdh from brain lysates of WT or Nedd4-2andi mice at age 4-weeks old. (B) Quantification of spontaneous spike rate (left) and representative raster plots of spontaneous spikes from 1-min recording (right) of WT or Nedd4-2andi cortical neuron cultures at DIV 13–14. (C) Quantification of average spontaneous spike amplitude (left) and representative average traces of 1-min recording (right) of WT or Nedd4-2andi cortical neuron cultures at DIV 13–14. The black lines represent the average of all the spikes within representative 1-min recordings. (D) Patch-clamp recording from WT or Nedd4-2andi cortical neurons at DIV 14. Representative mEPSC traces and quantification of mEPSC amplitude and frequency are shown (n = 15 for both WT or Nedd4-2andi neurons). Data are analyzed by Student’s t-test and represented as mean ± SEM with *p<0.05, **p<0.01.

Fig 1

doi: https://doi.org/10.1371/journal.pgen.1006634.g001