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Innate immunity mediated longevity and longevity induced by germ cell removal converge on the C-type lectin domain protein IRG-7

Fig 6

irg-7 is required for DAF-12 and DAF-16 activation in germline-less animals.

(A) Expression of an NLS-RFP reporter fused to the promoter of upstream of the F40F4.6 gene is induced in irg-7(zc6) mutants and upon germ cell removal (glp-1). Asterisks mark Student's T-test values of P<0.005 compared to the fluorescence in wild-type animals. 30 animals were analyzed per genotype. Error bars represent SE of 3 independent biological replicates. (B) F40F4.6 RNAi reduced the expression of the DAF-16 and DAF-12 reporters Psod-3::gfp and Pcdr-6::gfp in germline-less animals, but did not affect the expression of the PHA-4 and NHR-80 reporters Plgg-1::gfp and Pfat-6::gfp. Asterisks mark Mann-Whitney P values <0.0001 compared to the fluorescence in control RNAi-treated animals. 30 animals were analyzed per genotype. Error bars represent SE of 3 independent biological replicates. (C) F40F4.6 RNAi reduced DAF-16::GFP nuclear translocation in germline-less animals (left panels) but not in daf-2(e1370) mutants (right panels).

Fig 6

doi: https://doi.org/10.1371/journal.pgen.1006577.g006