Gene co-expression network connectivity is an important determinant of selective constraint
(A) Expression variance explained (R2) for local and distant eQTLs. Box plots show the maximum variance explained by a single eQTL for each gene and the total variance explained by all eQTLs for each gene. The widths of the boxes are proportional to the number of genes represented. The pairwise significiance of a Mann-Whitney test is indicated by asterisks: ***p < 0.001, **p < 0.01. (B) Broad sense heritability distributions for eGenes and non-eGenes. (C) Scatter plot showing the positions of all significant eQTLs in this study. No evidence of eQTL hotspots can be observed. Numbers indicate chromosome. (D) Genomic context of local and distant eSNPs. Context categories are normalized for feature length. When an eSNP overlapped with features on both strands, both of them were counted. For both local and distant eQTLs the features are based on the gene that is closest to the eSNP, and furthermore, for local eQTLs, the features are divided into whether the eSNP is located in or near the same gene that it is associated to (“associated gene”) or not (“other gene”). Flanking regions represent 2 kbp upstream and downstream from the gene. (E) Manhattan plots for local eQTLs (upper) and distant eQTLs (lower). Each point represents an eQTL.