XRN2 Autoregulation and Control of Polycistronic Gene Expresssion in Caenorhabditis elegans
Fig 5
rpl-43ICR is required while the operon promoter and the first gene are replaceable for XRN2 autoregulation.
(A) wild-type animals were exposed to mock or xrn-2 RNAi from L1 to L4 at 20°C. mRNA levels of ran-4 and R05D11.5 were quantified by RT-qPCR and normalized to act-1 mRNA levels with mock values defined as 1 (n = 3, means ± SEM). Values are shown in S1 Table. (B, D, F) Indicated reporter animals were exposed to mock or xrn-2 RNAi from L1 to L4 at 20°C and observed. GFP signal was detected in hypodermal (top), intestinal (middle, arrows) and vulval (bottom) cells. Positions of vulvae are indicated by square brackets. Corresponding DIC images of mid-L4 stage vulvae are shown (bottom). Scale bar: 100 μm. (C, E, G) Indicated reporter animals in bpnt-1(+) or bpnt-1(xe22) genetic background were cultured from L1 to L4 at 20°C and observed. GFP signal was detected in hypodermal (top), intestinal (middle, arrows) and vulval (bottom) cells. Images are shown as described in (B).