dBRWD3 Regulates Tissue Overgrowth and Ectopic Gene Expression Caused by Polycomb Group Mutations
Fig 8
dBRWD3 negatively regulates H3.3 at the promoter and 5' regions of Ubx and Abd-B in the trx over-expressing wing discs.
(A and B) A ChIP-qPCR analysis of dBRWD3-Flag levels at Ubx (A) and Abd-B (B). Black asterisks indicate negative control versus dBRWD3-Flag. Blue asterisks indicate negative control versus dBRWD3-Flag and trx over-expression. (C and D) A representative ChIP-qPCR analysis of HA-H3.3 levels at Ubx (C) and Abd-B (D) in the UAS-mCD8-GFP control, the trx over-expressing, and the trx over-expressing, dBRWD3 depleted wings as indicated. Black asterisks indicate trx over-expression versus trx over-expression and dBRWD3 depletion. (E) A ChIP-qPCR analysis of dBRWD3-Flag levels at Antp. Black asterisks indicate negative control versus dBRWD3-Flag. (F) A representative ChIP-qPCR analysis of HA-H3.3 levels at Antp. Black asterisks indicate control versus dBRWD3 depletion. ChIP-qPCR Data are shown as means ± S.D from 4 technical replicates. *, **, *** indicate P<0.05, 0.01, 0.001 respectively by Student's t-test.