Human Management of a Wild Plant Modulates the Evolutionary Dynamics of a Gene Determining Recessive Resistance to Virus Infection
Fig 4
Phylogeny of pvr2/eIF4E1 nucleotide sequence haplotypes and pvr2/eIF4E1-VPg:PVY interactions.
The presented phylogeny was reconstructed by the NJ method using pvr2/eIF4E1, coding sequences. Bootstrap values (1000 replicates) are indicated on the nodes. A to Q are pvr2/eIF4E1 haplotypes identified in chiltepin populations pvr1+ and pvr2+ to pvr225 denote pvr2/eIF4E1 alleles deduced from the pvr2/eIF4E1 coding sequences; pot1+: Potyvirus susceptibility allele from tomato (Solanum lycopersicum, accession number AY723733). pvr2/eIF4E1-VPg:PVY interactions were evaluated by yeast two-hybrid assays. Yeast growth (%) indicates the percentage of the yeast growth on the selective medium (-LWH) compared to the reference yeast colonies co-transformed with pGADT7::pvr2+ and pGBKT7::VPg-PVY; standard errors were obtained after 3 replicates of the yeast two-hybrid assays in which 3 independent colonies for each pvr2/eIF4E1-VPg:PVY combination were randomly selected.