The Polymerase Activity of Mammalian DNA Pol ζ Is Specifically Required for Cell and Embryonic Viability
Fig 4
Deletion of Polh does not ameliorate phenotypes caused by knockout of Rev3l.
(A) Genotypes of mouse pups produced by breeding parental Rev3l-/lox Polh-/—mice. (B) MEFs with the indicated genotypes were stained with DAPI, and for 53BP1 and γ-H2AX by immunofluorescence as in Fig 2 to detect foci of DNA double-strand breaks. The quantification shows the percentage of cells with >2 53BP1 and γ-H2AX foci in Rev3l-/lox Polh+/+, Rev3l-/lox Polh-/-, Rev3l-/Δ Polh+/+ and Rev3l-/Δ Polh-/- MEFs 9 days after AdCre treatment. (C) Survival of primary MEFs as in part B, 120 hr after addition of cisplatin, as measured by ATP content. For panels B& C, Data represent mean ± SEM.