Drosophila Nipped-B Mutants Model Cornelia de Lange Syndrome in Growth and Behavior
Fig 3
Additional myc (dm) expression rescues adult wing size of Nipped-B407 heterozygous mutants and increases apoptosis in wild-type 3rd instar wing imaginal discs.
(A) The box plots indicate the distribution of the wing blade areas of the indicated genotypes, where tub-myc indicates a single copy of the tub-myc transgene that expresses Drosophila dm from a minimal tubulin gene promoter. The increases in wing area induced by tub-myc are significant for both the P57B / + and Nipped-B407 / + genotypes (p<0.0001, t test). Cell numbers and sizes were also determined for each genotype and are given in Table 3. (B) Examples of TUNEL staining of late 3rd instar wing imaginal discs of the indicated genotypes to visualize apoptotic cells. (C) Boxplot distributions of the number of apoptotic cells in 3rd instar wing discs. The number of apoptotic cells in P57B / + discs with the tub-myc transgene is significantly higher than the other genotypes (p<0.0001, student t test).