Pelle Modulates dFoxO-Mediated Cell Death in Drosophila
Fig 8
dFoxO is required for loss-of-pll induced bristle phenotype on notum.
(A-G) Light micrographs showing bristles on the adult notum. Compared with the pnr-Gal4 control (A), RNAi-mediated depletion of pll in notum resulted in reduced bristle number (B), which was suppressed by knocking-down dFoxO (D), deleting one or both copies of endogenous dFoxO (E and F), or expressing Pll (G), but not by expressing a GFP RNAi that served as a negative control (C). The lower panels show high magnification view of the boxed areas in upper panels. (H) Quantification of bristles number in the boxed areas from A-G. One-way ANOVA with Bonferroni multiple comparison test was used to compute P-values, significance is indicated with asterisks (*** P<0.001, ** P<0.01). ns stands for not significant. Detailed genotypes: (A) pnr-Gal4/+ (B) UAS-pll-IRV2889/+; pnr-Gal4/+ (C) UAS-pll-IRV2889/+; pnr-Gal4/UAS-GFP-IR (D) UAS-pll-IRV2889/+; pnr-Gal4/UAS-dFoxO-IR (E) UAS-pll-IRV2889/+; pnr-Gal4/dFoxOΔ94 (F) UAS-pll-IRV2889/+; pnr-Gal4 dFoxOΔ94/dFoxOΔ94 (G) UAS-pll-IRV2889/+; pnr-Gal4/UAS-Pll.