Protein O-Glucosyltransferase 1 (POGLUT1) Promotes Mouse Gastrulation through Modification of the Apical Polarity Protein CRUMBS2
Fig 6
Indistinguishable gastrulation defects in Poglut1wsnp and Crumbs2-/- embryos.
(A-C) Transverse sections through the primitive streak of early bud (E7.5) wild-type (A), Poglut1wsnp (B) and Crumbs2-/- (C) embryos immunostained for laminin (green) and E-Cadherin (red). The primitive streak (right) is marked by the break in the laminin-containing basement membrane (vertical bar). The mesodermal wings between the two laminin-containing basement membranes surround nearly the entire embryo in all three genotypes. (D-F) Transverse sections through E8.0 wild-type (D), Poglut1wsnp (E) and Crumbs2-/- (F) embryos at E8.0 (D, E, F). By E8.0 the mutants have less mesoderm (compare the head mesenchyme in WT and mutants, asterisks). am = amnion. D’, E’ and F’ and D”, E” and F” are higher magnification of the primitive streak (boxed in D, E, F) showing laminin expression (D’, E’ and F’) and E-Cadherin expression (D”, E” and F”). Both mutants have a broader streak as defined by the region of laminin breakdown. Both Poglut1wsnp and Crumbs2-/- mutants show an accumulation of E-cadherin+ cells in the vicinity of the primitive streak. Scale bars: A to F = 20 μm and D’ to F” = 40 μm.