Identification of Astrotactin2 as a Genetic Modifier That Regulates the Global Orientation of Mammalian Hair Follicles
Fig 3
Origin of the Astn2 exon 5 deletion.
(A) PCR analysis of genomic DNA in the neighborhood of Astn2 exon 5, as shown in Fig 2E. The ~30 kb deletion is present in 129X1/SvJ mice and R1 ES cells, but is absent from C57Bl6/J, 129S1/SvlmJ, and 129S6/SvEvTac mice. (B) The Astn2 locus from the three 129 mouse lines shown in (A) was bred to homozygosity in a Fz6-/- background. The ridge phenotype was observed only in the presence of the 129X1/SvJ Astn2 locus; mice were photographed at P14.