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Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress

Fig 1

Phylogenetic clustering of the Arabidopsis thaliana CRK group of RLKs and summary of the crk T-DNA insertion collection.

(A) The coding region of the CRKs of Arabidopsis thaliana (including the truncated CRK9 At4g23170 and the putative pseudogene CRK35 At4g11500) was aligned using Muscle. The maximum-likelihood phylogenetic tree was estimated in MEGA6 using all sites (no gap penalty). The initial guide tree was constructed using maximum parsimony. Values at branch nodes represent bootstrap values (1000 replicates). CRK43 (At1g70740), CRK44 (At4g00960) and CRK45 (At4g11890) lack signal peptide, CRK ectodomain (ED) and transmembrane domain. (B) Information on T-DNA insertion lines for corresponding crk mutants is summarized: location of the T-DNA insertion in the gene (detailed information in S3 Fig), number of T-DNA insertions per line (determined by quantitative PCR; S1 Table) and transcript level of the corresponding crk mutant (according to semi-quantitative RT-PCR and qPCR; detailed information in S1 Table). For two additional crk10 alleles (crk10-1 and crk10-3) information can be found in S1 Table.

Fig 1

doi: https://doi.org/10.1371/journal.pgen.1005373.g001