Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress
(A) Polysome profiles under normal and oxidative stress conditions. (B) SufI synthesis in an in vitro translation system, with or without oxidative stress. The SufI gene fused with a C-terminal His6-tag sequence constructed in a pET-28b plasmid (Novagen) was used as a template in an in vitro translation reaction. The protein product was detected by western blotting with an anti-His6 antibody. (C) Detergent-insoluble proteins under normal (N) and oxidative stress (OS) conditions. Insoluble proteins extracted from E. coli BL21(DE3) and wild-type BW25113 cells grown at 47°C for 10 min were used as positive controls (Heat). Soluble proteins (Sup) were loaded on the same gel as controls. (D) Western blot analysis of the molecular chaperones GroEL and DnaK in E. coli BL21(DE3) and wild-type BW25113 cells grown under normal (N) conditions, oxidative stress (OS) condition, or heat stress (heat) at 47°C for 30 min.