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Transfer RNAs Mediate the Rapid Adaptation of Escherichia coli to Oxidative Stress

Fig 1

Optimization of the oxidative stress conditions.

(A) Cell growth in the presence of different concentrations of H2O2. E. coli BL21(DE3) cells were cultivated in M9 minimal medium. Cell growth in the presence of 0.25–1 mM H2O2 was monitored by measuring OD600 values. (B) Cell viability assay using propidium iodide staining and flow cytometry. Histograms showing cells exposed to 0.5 mM H2O2 for 15, 30, 60, or 90 min were obtained (cyan lines). Cells grown under normal condition was used as a positive control (black line), and cells killed by incubation at 65°C for 15 min were used as a negative control (red line).

Fig 1

doi: https://doi.org/10.1371/journal.pgen.1005302.g001