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Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant

Fig 8

AtRAD51 and AtDMC1 could play different roles in the synaptic process.

(A-J) Dual immunolocalization of AtASY1 (green) and AtZYP1 (red) on (A) WT, (B) Atfas1-4, (C) Atspo11-1-5, (D) Atfas1-4 Atspo11-1-5, (E) Atdmc1-2, (F) Atfas1-4 Atdmc1-2, (G) Atrad51-3, (H) Atfas1-4 Atrad51-3, (I) Atrad51-2 and on (J) Atfas1-4 Atrad51-2. Dual immunolocalization of AtASY1 (green) and AtDMC1 (red) on (K) Atrad51-3, (L) Atrad51-2, (M) Atfas1-4 Atrad51-3, and on (N) Atfas1-4 Atrad51-2. Bars = 5 ┬Ám. (O) Dispersion diagram for the total foci per nucleus in Atrad51-3 (blue), Atrad51-2 (red), Atfas1-4 Atrad51-3 (green), and Atfas1-4 Atrad51-2 (purple) PMCs are indicated. Each dot is the count from a single nucleus. P values are from two-sided Wilcoxon Mann-Whitney tests (**P < 0.01; ***P < 0.001).

Fig 8