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Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant

Fig 6

Atfas1-4 Atrad51 genetic analysis. (A-D) Atrad51-3 KO mutant plants.

Homologous chromosomes do not pair, and severe chromatin fragmentation is observed. (E-H) Atfas1-4 Atrad51-3. Certain regions appear to be paired (arrow) at post-leptotene and some associated chromosomes are bioriented at metaphase I (arrow). (I-L) Atrad51-2 KD mutant plants display some short SC stretches (arrow) and chromosome associations at metaphase I (arrow). (M-P) The double mutant Atfas1-4 Atrad51-2 also shows short SC stretches (arrows) at post-leptotene and bioriented associated chromosomes at metaphase I (arrow). Bars = 5 ┬Ám.

Fig 6