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Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant

Fig 5

DSB formation in Atfas1-4, Atspo11-1-5, and in the double mutant Atfas1-4 Atspo11-1-5.

(A) Dual immunolocalization of AtASY1 (green) and γH2AX (red) in Atfas1-4 Atspo11-1-5. Bar = 5 µm. (B) Quantification of γH2AX foci in the different backgrounds. Each dot is the count from a single nucleus. P values are from two-sided Wilcoxon Mann-Whitney tests (***P < 0.001).

Fig 5