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Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant

Fig 4

Atfas1-4 Atspo11-1-5 and Atfas1-4 Atdmc1-2 display CO on some occasions.

Meiotic spreads of PMCs stained with DAPI. (A-D) Atspo11-1-5. (F-I) Atfas1-4 Atspo11-1-5. (K-L) Atdmc1-2. (P-S) Atfas1-4 Atdmc1-2. (E, J, O, T) FISH metaphases I using 5S (red) and 45S (green) rDNA probes for chromosome identification in these four backgrounds. Bivalents are observed in the two double mutants (arrows). Bars = 5 ┬Ám.

Fig 4

doi: https://doi.org/10.1371/journal.pgen.1005301.g004