Analysis of the Relationships between DNA Double-Strand Breaks, Synaptonemal Complex and Crossovers Using the Atfas1-4 Mutant
(A, B) FISH using 5S (red) and 45S (green) rDNA probes for chromosome identification and chiasma scoring. (A) WT metaphase I. (B) Atfas1-4 metaphase I. Four ring bivalents (with at least one chiasma in both arms) and one rod bivalent (chromosome 3) are observed. (C, D) Two different examples for the GC test loci using NFTL. (C) Tetrad without GC (2:2). (D) Tetrad with GC (3:1). Bars = 5 µm.