Notch Is Required in Adult Drosophila Sensory Neurons for Morphological and Functional Plasticity of the Olfactory Circuit
Fig 5
The source of the ligand for N reporter activity in ORNs is PNs.
(A) Schematic of experimental protocol. One day old females were exposed to GA, EB or oil for 4 days and then analyzed. The protocol for flies carrying GAL80ts was as in Fig 2B. Flies expressing UAS.DTI [56] (lane 11) were aged for one week prior to odor exposure. It has been shown that cell bodies and axons of ORNs expressing DTI are undetectable 5 days after eclosion [49]. (B) Diagram of the olfactory circuit depicting N reporter activation in VA6 ORNs in response to exposure to GA. Dl is being removed from VA6 PNs. C. N-LV LexOP.dGFP females carrying either UAS.Dl shRNA (lanes 1–5), UAS.DlXK8 long inverted repeat RNA (lanes 6 & 7), UAS.aph-1 shRNA (lane 8), UAS.Dl and UAS.neur (lane 10), UAS.epsin RNAi (lane 9), or UAS.DTI [56] (lane 11) in the indicated cell type were exposed to a 1:100 dilution of GA (VA6), EB (VM2) or oil for 4 days. In lanes 3, 6, 7 & 10 the flies also carried tubP-gal80ts to limit expression to adults, and in lanes 6, 7 & 9 the flies also carried UAS.dicer-2 (dcr) to potentiate the RNAi. The level of N reporter activity seen in odor exposed flies carrying the indicated transgenes was normalized to the N reporter activity seen in odor exposed controls. The normalized activity of the odor exposed flies is presented. The entire data set comprising both odor exposed and air exposed flies is presented in S2 Fig. The following GAL4 drivers were used: VA6 PNs, MZ612; VM2 PNs, NP5103; VA6 glia, repo; VA6 and VM2 LNs, NP2426; VA6 ORNs, Or82a-GAL4.