SYD-1C, UNC-40 (DCC) and SAX-3 (Robo) Function Interdependently to Promote Axon Guidance by Regulating the MIG-2 GTPase
Fig 5
MIG-2 functions with SAX-3 to regulate axon guidance.
HSN guidance defects were observed in mig-2 null mutants and in sax-3 null mutants. HSN guidance defects were not enhanced in sax-3 mig-2 double null mutants, indicating that SAX-3 and MIG-2 function together to regulate HSN axon guidance. The mig-2 null mutant enhances HSN guidance defects in unc-40 null mutants. Alleles are syd-1 (ju2), mig-2(ok2273), sax-3(ky123), unc-40(e1430). HSN axon guidance was scored as defective if the axon failed to reach the ventral nerve cord. For all experiments, n≥200. Brackets indicate statistically significant difference, Z test for proportions (***p<0.0001).