The Escherichia coli Small Protein MntS and Exporter MntP Optimize the Intracellular Concentration of Manganese
Fig 3
MntS helps activate the manganese-dependent RNR, NrdEF.
Cells pre-cultured in anaerobic MOPS glucose/casamino acids medium were diluted into aerobic medium with or without 50 μM MnCl2 at time zero; viability was monitored by anaerobic plating. “Import-minus” strains contain ΔtonB ΔfeoABC ΔzupT null alleles and therefore have reduced iron import. The data are representative of at least three independent experiments. A. Strains were JEM609 (Import-), JEM1181 (Import- ΔmntS), JEM1136 (Import- ΔnrdAB), JEM1836 (Import- ΔnrdAB ΔmntS), and JEM722 (Import- ΔnrdAB ΔnrdHIEF). B. Viability of Import- ΔnrdAB ΔmntS (JEM1183) harboring empty vector (pACYC184) or pMntS2 (pLW131, mntS under the control of its native promoter).