Contribution of the Two Genes Encoding Histone Variant H3.3 to Viability and Fertility in Mice
Fig 7
Developmental potential of H3f3b mutant zygotes, and H3f3a, H3f3b double-mutant follicles.
(A) Presumptive H3f3b-/- ova were flushed from the oviducts of experimental females at 1½ dpc. Some 2-cell ova were obtained, with one shown at top-left. (B) left panel, ovary of an experimental H3f3ac/c, H3f3bc/c, 0/Tg(Zp3-cre) female at 10 wk, in which oocytes are of presumptive genotype H3f3a-/-, H3f3b-/-; right panel, control 10 wk ovary showing prominent Graafian follices (Gf), and copora lutea (cl). Bars, 0.5 mm, (C) top panel, 10 wk experimental ovary showing resting primordial follicles (pf), and early-stage growing primary oocytes (po). Degenerating primary oocytes are indicated (arrows); bottom panel, experimental 10 wk ovary stained with PAS to show the zona pellucida (pink, arrows). Scale bars, 50 μm.