Overlapping and Non-overlapping Functions of Condensins I and II in Neural Stem Cell Divisions
Figure 5
Condensin II prevents hyperclustering of chromocenters in the interphase nucleus in NSCs.
(A) Shown here is a schematic view of M. musculus chromosomes and an interphase nucleus. Pericentric heterochromatin derived from non-homologous chromosomes associate with each other to form clusters within the interphase nucleus. These structures are referred to as chromocenters. (B) Frozen sections of embryonic brains at E16.5 were immunolabeled with an antibody against histone H3 trimethylated at lysine 9 (H3K9me3) along with Hoechst staining. Shown on the top are wide areas containing the VZ. The lower panels indicate close-up views of the nuclei indicated by the white rectangles. The data shown are from a single representative experiment out of three repeats. Sections from three different embryos of each genotype were analyzed. Maximum intensity projections are shown. Bars, 10 µm. The numbers of chromocenters per nucleus in the VZ were measured and plotted. Data were obtained from 50 nuclei in the VZ. *** P<0.001 (t-test with a Holm correction for multiple comparisons). (C) Frozen sections of embryonic brains at E16.5 were immunolabeled with an antibody against nucleolin along with Hoechst staining. Wide and close-up views are shown as in (B). The data shown are from a single representative experiment out of three repeats. Sections from three different embryos of each genotype were analyzed. Maximum intensity projections are shown. Bars, 10 µm. The numbers of nucleoli per nucleus in the VZ were measured and plotted. Data were obtained analyzed as in (B).