Functional Diversity of Carbohydrate-Active Enzymes Enabling a Bacterium to Ferment Plant Biomass
A Variation in cleavage activities of GH10 enzymes on xylan. B GH5 and C GH26 family members differ in their activities and substrate specificities on amorphous cellulose (red), glucomannan (green), xyloglucan (violet), galactomannan (yellow), mannan (gray). Enzyme activities in A–C are nmol reducing sugar released per milligram enzyme per minute. D–G CAZyme mixtures have higher activities than the individual enzymes. D Cphy1163 and Cphy3367 alone and together on amorphous cellulose. E Cphy2105, Cphy3009, and Cphy3207 alone and the latter two enzymes plus Cphy2105 on xylan. F Cphy1719 and Cphy1071 alone and together on glucomannan. G Cphy1687, Cphy2567, and Cphy3310 alone and the latter two enzymes plus Cphy1687 on homogalacturonan. In D–G, enzyme activities are shown as reducing sugar (nmol) produced by individual and combined enzymes. The fraction of the reducing sugar produced by the mixed enzymes that exceeds the sum of the individual enzymes is shown in green.