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Functional Diversity of Carbohydrate-Active Enzymes Enabling a Bacterium to Ferment Plant Biomass

Figure 4

Cleavage A, binding B, and CAZy database classification C of purified enzymes.

A Polysaccharide cleavage was quantified as nmol reducing sugar released per milligram enzyme per minute: >160 red, 80–160 orange, 40–80 yellow, 20–40 green, <20 gray. B Binding to insoluble polysaccharides was quantified as percentage enzyme bound to substrate: >30% red, 20–30% orange, 15–20% yellow, 10–15% green, <10% gray. C CAZy database classifications: glycoside hydrolases (GH), carbohydrate esterases (CE), polysaccharide lyases (PL), and carbohydrate binding domains (CBM). Among 56 purified CAZymes, only the 32 enzymes for which activities were found are shown.

Figure 4