Differential Responses to Wnt and PCP Disruption Predict Expression and Developmental Function of Conserved and Novel Genes in a Cnidarian
In situ hybridization analysis of Wnt3-MO-embryo over-expressed transcripts in early gastrula, 24hpf planula and 48hpf planula stages (from left to right in each panel). The 18 transcripts with the highest z-scores were analyzed. Seven genes showed expression clearly restricted to the aboral territories at planula stages (A-G), already apparent at the early gastrula stage except in in the case of Dkk (C). Two additional transcripts showed an aboral expression pattern but with additional signal in endodermal region of planula larvae: ZpdA (H) and Botch2 (I). 9 transcripts (J-R) showed low ubiquitous or undetectable expression at the gastrula stage followed by a variety of patterns in planula larva including ubiquitous (Q-R), mainly endodermal (K-O) and/or with diverse dynamic distributions of scattered cells in the ectoderm and/or endoderm (J, M, N, O, P). We classified the expression profiles as “Aboral-type” (A-G, panels outlined in green), “Delayed” type (L-R, panels outlined in black) or as showing a mixture of these profiles (4 remaining panels). Representative images from at least three experiments are shown. All embryos are oriented with the oral pole uppermost. Bottom right: gene name (see Table 1). Scale bar 50 µm.