Differential Responses to Wnt and PCP Disruption Predict Expression and Developmental Function of Conserved and Novel Genes in a Cnidarian
In situ hybridization analysis of Wnt3-MO-embryo under-expressed transcripts in early gastrula, 24hpf planula and 48hpf planula stages (from left to right in each panel). The expression of Wnt3 (A; Momose et al., 2008) is compared with that of the 20 down-regulated transcripts with the highest z-scores. All genes showed heavily oral-biased expression at the early gastrula stage, with two principle distribution patterns: Predominantly in the oral ectoderm at all stages (panels A-I; “Oral” expression pattern, outlined in red); Mainly in ingressing/ingressed cells during gastrulation and then in the endodermal region of the planula (panels J-U; ‘Ingressing/Endodermal” expression pattern, outlined in orange). Two of the “Oral pattern” transcripts (panels H and I) also showed expression in the endoderm at the planula stage. Representative images of the patterns observed in at least three experiments are shown. All embryos are oriented with the oral pole uppermost. Bottom right: gene name (see Table 1). Scale bar 50 µm.