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The Drosophila melanogaster Phospholipid Flippase dATP8B Is Required for Odorant Receptor Function

Figure 3

dATP8B is expressed and required in Or-expressing olfactory receptor neurons.

(A–A′) dATP8B protein localises to the dendrites of ORNs. 14 µm thick antennal sections from wild type flies (CS-5) were stained for anti-dATP8B (magenta). Strong anti-dATP8B staining is seen in the shafts of the sensilla (the location of the outer dendrites) of basiconic (arrows) and trichoid (arrowhead) sensilla. Staining is also seen in the inner dendrites and cell bodies, however this staining is also present in dATP8B mutants. (B) The strong outer dendrite staining of anti-dATP8B is absent in dATP8B mutants (ll2/Df(3R)Exel8155), indicating it is specific for dATP8B. (C) dATP8B and Orco co-localise in the outer dendrites. 14 µm thick antennal sections from wild type flies were stained for anti-dATP8B (magenta) and Orco (green). (D) dATP8B is absent from the outer dendrites of Gr21a-expressing (ab1C) neurons. 14 µm thick antennal sections from Gr21a>mCD8:GFP flies were stained for anti-dATP8B (magenta) and anti-GFP (green). (E) In Orco-GAL4: UAS-dATP8BRNAi flies (red bars) the EAG response is significantly reduced compared to controls (black and grey bars, t-test, Bonferroni, p<0.05) for some of the same odorants that are affected by the two dATP8B mutant alleles. Bars represent mean EAG responses ± SEM (n = 6–10). Odorants are: EA, ethyl acetate, PA, pentyl acetate, MS, methyl salicylate, OL, 1-octen-3-ol, HB, ethyl 3-hydroxybutanoate, EH, ethyl hexanoate, BZ, benzaldehyde, PO, paraffin oil (solvent blank).

Figure 3

doi: https://doi.org/10.1371/journal.pgen.1004209.g003