Selective Disruption of Aurora C Kinase Reveals Distinct Functions from Aurora B Kinase during Meiosis in Mouse Oocytes
Figure 1
AURKB is expressed in mouse oocytes.
(A) GV-intact oocytes were collected from CF1 mice and matured in vitro for 8 h (Met I), or 16 h (Met II), prior to fixation and staining with an anti-AURKB antibody. (B) GV-intact oocytes were collected from WT and Aurkc−/− mice and matured in vitro for 16 h (Met II), prior to fixation and staining with an anti-AURKB antibody. Merged images show AURKB in green and DNA in red. (C) GV-intact oocytes were collected from WT, Aurkb−/−, and Aurkc−/− mice, microinjected with the indicated cRNA, and matured in vitro for 16 h (Met II), prior to fixation and staining with an anti-Survivin antibody. Merged images show AURKB-GFP in green, Survivin in red, and DNA in blue. These experiments were conducted with a minimum of 20 oocytes for each group. Shown are representative images (Scale bar, 10 µm). (D) 20 GV-intact oocytes were collected from CF1 mice and microinjected with the indicated cRNA. Two hours after injection, the oocytes were matured to Met II in vitro (16 h). The total numbers of non-injected control oocytes (Non-inj.) are indicated in parenthesis. Total cellular lysates were probed with the indicated antibody. The panels are images of the same membrane that was stripped and re-probed. The arrows indicate the specific AURKB protein band, and the asterisk indicates a presumed degradation product of AURKB-GFP.