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GC-Rich DNA Elements Enable Replication Origin Activity in the Methylotrophic Yeast Pichia pastoris

Figure 2

The GC-ACS is required for GC-ARS function.

Wild type (WT) and mutant (MUT) alleles of the twelve ARSs indicated were cloned into a URA3 ARS-less vector and used to transform ura3 yeast on selective medium plates lacking uracil. Plates were grown at 30°C for five days before pictures were taken. Colony formation indicates plasmid maintenance and ARS activity. The GC-ACS was positioned <15 bp away from the 5′ endpoint in all ARS sequences. The sequences of the fragments tested are listed in Table S4.

Figure 2

doi: https://doi.org/10.1371/journal.pgen.1004169.g002