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Developmental Link between Sex and Nutrition; doublesex Regulates Sex-Specific Mandible Growth via Juvenile Hormone Signaling in Stag Beetles

Figure 2

Developmental staging chart of prepupal development from the larval-prepupal transition to pupation.

Mandibular growth (cell proliferation) is indicated on the y-axis over developmental time in days on the x-axis (hashmark = 1 day). Three distinct forms are recognized - the last instar larva, the prepupa, and the pupa. Known landmarks of mandibular proliferation in large males are indicated by the red circles and shown in diagram form below the graph [5]. The end of the larval period and the initiation of the prepupal period is indicated by Pupal Cell Construction (PCC) and is defined as the starting point of prepupal Stage 1. Outwardly the larva does not change its morphology. Stage 1 lasts approximately 2 days until the initiation of the first Gut Purge (GP) in which the prepupa begins to transform and empties out half of its gut contents. The time the prepupa spends in the first GP is known as Stage 2. Stage 3 is a quiescent phase where the prepupa undergoes massive adult imaginal tissue proliferation but outwardly appears suspended in the first GP. Stage 4 occurs over only a few hours and begins with the second GP in which the last remnants of the gut contents are egested and the prepupa completely metamorphoses into the pupa.

Figure 2

doi: https://doi.org/10.1371/journal.pgen.1004098.g002