A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool
(A–C) Relative growth rate (compare to wild-type) of the following five deletion strain: tL(GAG)G (blue), tR(CCU)J (red), tiM(CAU)C (green), tH(GUG)G1 (magenta) and tR(UCU)M2 (cyan). Strains were grown in media supplemented with increasing concentrations of the following proteotoxic agent: AZC (A) Tunicamycin (B) DTT (C). (D) Percentage of cells that contain puncta in the populations of the above strains. (E) Percentage of cells that contain puncta in the populations of the above strains following treatment with 2.5 mM AZC. Data are presented as mean of 3 biological repetitions +/− SEM, in each repetition 500 cells were counted. (*) P<0.001 by Students t-test. (F–G) Images of representative fields for the wild-type and tR(CCU)J deletion strain, without treatment (F) and following treatment with 2.5 mM AZC (G).