A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool
(A–B) Relative growth yield values of the tRNA deletion library strains in rich medium (A) and low glucose (B), sorted by anti-codon and amino-acid identity along the x-axis. Each dot along the vertical lines denotes the value (data are represented as mean of 3 biological repetitions +/− SEM) of a deletion strain of different tRNA gene of the respective family. The horizontal lines mark two standard deviations around the mean of the wild-type. Dots above or below these lines are considered non-normal phenotypes (see also Supplemental figure S7). (C) Relative growth yield values (data are presented as mean of 3 biological repetitions +/− SEM) of various double deletion combinations consisting of: five tR(UCU) family members, tR(CCU) and trm9 deletion strains as indicated on the x-axis, along with the five members of the tR(UCU) family each denoted by a different shape and color in the legend. (D) Relative growth yield of the five tR(UCU) members across different growth conditions, indicated on the x-axis. (E) Enrichment of conserved elements in tRNA genes divided according to phenotype observed in rich media for each growth parameter. Each column in the matrix denotes a conserved element as defined by . Color bar indicates the −log10 of the hypergeometric p-value. (F) log10 E-value found by the MEME software for the most significant motif in a 9 bp window starting from the position indicated by the x-axis. The LOGOs of the two significant motifs are displayed below, next to a number indicating its position. Position 0 is the first position of the mature tRNA.