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A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool

Figure 4

Differential contribution of identical tRNA gene copies.

(A–B) Relative growth yield values of the tRNA deletion library strains in rich medium (A) and low glucose (B), sorted by anti-codon and amino-acid identity along the x-axis. Each dot along the vertical lines denotes the value (data are represented as mean of 3 biological repetitions +/− SEM) of a deletion strain of different tRNA gene of the respective family. The horizontal lines mark two standard deviations around the mean of the wild-type. Dots above or below these lines are considered non-normal phenotypes (see also Supplemental figure S7). (C) Relative growth yield values (data are presented as mean of 3 biological repetitions +/− SEM) of various double deletion combinations consisting of: five tR(UCU) family members, tR(CCU) and trm9 deletion strains as indicated on the x-axis, along with the five members of the tR(UCU) family each denoted by a different shape and color in the legend. (D) Relative growth yield of the five tR(UCU) members across different growth conditions, indicated on the x-axis. (E) Enrichment of conserved elements in tRNA genes divided according to phenotype observed in rich media for each growth parameter. Each column in the matrix denotes a conserved element as defined by [42]. Color bar indicates the −log10 of the hypergeometric p-value. (F) log10 E-value found by the MEME software for the most significant motif in a 9 bp window starting from the position indicated by the x-axis. The LOGOs of the two significant motifs are displayed below, next to a number indicating its position. Position 0 is the first position of the mature tRNA.

Figure 4