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A Comprehensive tRNA Deletion Library Unravels the Genetic Architecture of the tRNA Pool

Figure 3

Extensive redundancy underlies robustness to tRNA gene deletion.

(A) Schematic representation of the genetic interactions within and between tRNA families. Families are denoted by dark grey circles and grouped (black dashed line) according to their tRNA copy number. Each family is denoted by its anti-codon and amino-acid. A protein-coding gene i.e. TRM9 is denoted by a grey box. Each filled circle indicates a tRNA deletion strain. The lines connecting the deletion strains denote a co-deletion of these genes (a multi-tRNAs deletion strain). The color of the filled circles and lines denote the severity of the growth phenotype for the respective strain: blue for normal growth, purple for impaired growth (worse than wild-type) and red for lethality. (B) Epistasis values for multi-tRNAs deletion strains which contain the deletion of tL(GAG) and either: one tL(UAG) gene, two tL(UAG) genes, tL(CAA) (which is a tRNA of different Leucine family), and tW(CCA) (which is a non-Leucine tRNA) as controls. (C) Epistasis values for multi-tRNAs deletion strains which contain the deletion of trm9 with: the singleton tR(CCU), and tR(ACG) which is a tRNA of different Arginine family and tW(CCA) which is a non- Arginine tRNA as controls. In both (B) and (C) epistasis values of the relative growth yield and growth rate are indicated in grey and green respectively. Data is presented as mean of 3 biological repetitions +/− SEM.

Figure 3

doi: https://doi.org/10.1371/journal.pgen.1004084.g003