Base Pairing Interaction between 5′- and 3′-UTRs Controls icaR mRNA Translation in Staphylococcus aureus
(A) Schematic representation of the icaR mRNA molecule, mapped by RACE, showing the length of the 5′-UTR, ORF and 3′-UTR. The nucleotide sequence of the 3′-UTR is shown. The sequence of the transcriptional terminator is underlined and its secondary structure, predicted by Mfold, is shown. (B) Northern blots carried out with two different riboprobes, one targeting the icaR ORF region and the other, the icaR mRNA 3′-UTR.