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A Global In Vivo Drosophila RNAi Screen Identifies a Key Role of Ceramide Phosphoethanolamine for Glial Ensheathment of Axons

Figure 5

CerPE is very essential for axonal ensheathment by wrapping glia.

(A) RNAi against Spt-I, schlank, Des1 and Pect were expressed in wrapping glia (Nrv2-GAL4). Merged projections of the peripheral nerves along with an orthogonal projection are presented. GFP (green) was used to label the wrapping glial membrane, and HRP (red) and repo (blue) were used to label the neuronal membrane and glial nuclei, respectively. (B) TEM micrographs of L3 larval peripheral nerve cross-sections are shown. Wrapping glia is color-coded. Axonal ensheathment is incomplete upon loss of lace function in wrapping glia. Proper ensheathment of axons is observed only in control (+). Scale 1 mm. (C) Quantification of GFP signal intensity of wrapping glial membrane showed a reduction of GFP signal intensity. (D) Quantification of the number of unwrapped axons. One-way ANOVA followed by Dunnett post hoc test was performed. All graphs represent mean values 6 SD. * p,0.05, ** p,0.01, *** p,0.001.

Figure 5