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A Global In Vivo Drosophila RNAi Screen Identifies a Key Role of Ceramide Phosphoethanolamine for Glial Ensheathment of Axons

Figure 4

Glia require CerPE for axonal ensheathment.

(A) A genetic dissection study of sphingolipid biosynthetic pathway shows that glial knockdown of Spt-I, schlank, Des1 and Pect result in swelling and wrapping defects (repo-GAL4) similar to the lace phenotype. Merged projections of confocal stacks are presented. Scale 20 µm. (B) Sphingolipid biosynthesis pathway. (C) RNAi against these 4 genes were expressed in neurons by using elav-GAL4 driver line. HRP (red) is used to visualize the membrane morphology of the neurons. Scale 20 µm. (D) Quantification (mean± SD) showed that the swellings were only observed upon glial specific knockdown but not in neuron-specific knockdown.

Figure 4

doi: https://doi.org/10.1371/journal.pgen.1003980.g004