A Global In Vivo Drosophila RNAi Screen Identifies a Key Role of Ceramide Phosphoethanolamine for Glial Ensheathment of Axons
(A) Scheme of the screening strategy. (B) Pre-screening result with nejire RNAi with tub-GAL80ts; repo-GAL4. Survival curves were analyzed with Log-Rank Mantel Cox test. p<0.0001 (C) Results from the primary screening reveal 861 RNAi lines with lethality and 30 RNAi lines with impaired locomotion. (D) Peripheral nerves of L3 larval PNS, glial membrane (green) swelling and axonal (red) wrapping defect were observed upon knockdown of lace with 2 different RNAi lines (Transformant ID 21803, 110181). repo>mCD8-GFP/+ served as a control. Merged projection of confocal z-stacks is presented. In insets, orthogonal sections of the nerve region marked by a white line are shown (E) Quantification of the average cross-section of the peripheral nerves after glial specific knockdown of lace. (F) Merged projection of confocal z-stacks did not reveal any visible morphological changes in the neuronal morphology in the PNS after knockdown of lace specifically in the neurons (HRP). (G) Quantification of the average cross-section of the peripheral nerves after neuron-specific knockdown of lace. For the statistical analysis, unpaired t-test was performed with the respective control. Scale bar 20 µm. ns not significant. All graphs represent mean values ± SD. ** p<0.01 *** p<0.001.