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Elevated Mutagenesis Does Not Explain the Increased Frequency of Antibiotic Resistant Mutants in Starved Aging Colonies

Figure 1

Actual number of observed mutations in rifampicin resistant and nalidixic acid resistant starved genomes is much smaller than expected within HMS (hypermutating cell subpopulation) cells.

Drawn is the distribution of the numbers of mutations we would expect to find in 1000 simulated experiments in which we sequence 15 HMS genomes, and each HMS genome is expected to accumulate on average 4.82 mutations (calculated based on a 25-fold increase in mutagenesis across the entire starved population and an HMS size of 10%). The arrows represent the numbers of mutations we actually observed in 15 fully sequenced starved rifampicin (Red), or nalidixic acid (Blue) resistant genomes.

Figure 1