FliZ Is a Global Regulatory Protein Affecting the Expression of Flagellar and Virulence Genes in Individual Xenorhabdus nematophila Bacterial Cells
Figure 2
Bimodal expression of flagellin and hemolysin genes in Xenorhabdus.
The strains indicated were grown in LB medium to mid-exponential growth phase (strains with PfliC-gfp[AAV], pPROBE-gfp[AAV] constructs or PD31-gfp[AAV]constructs) or mid-stationary growth phase (strains with PxaxAB-gfp[AAV] or PxhlBA-gfp[AAV] constructs) and GFP fluorescence signals were quantified in individual bacterial cells by flow cytometry. The pPROBE-gfp[AAV] vector carrying a promoter-less gfp gene and the PD31-gfp[AAV] construct carrying the gfp gene under the control of a constitutive promoter were used as negative and positive controls, respectively. Data are shown on two-dimensional dot plots, with the GFP signal on the x-axis and the forward scatter parameter (FSC) on the y-axis. Gates corresponding to GFP-negative and GFP-positive populations are shown and the corresponding percentages are indicated at the bottom left and bottom right of each image, respectively.