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The Highly Prolific Phenotype of Lacaune Sheep Is Associated with an Ectopic Expression of the B4GALNT2 Gene within the Ovary

Figure 4

B4GALNT2 transferase activity revealed by DBA lectin and KM694 antibody staining in Lacaune sheep ovary.

Photomicrographs of ovarian sections from +/+ and L/L ewes and stained either with biotinylated-DBA lectin (500 ng/ml) or KM694 mouse monoclonal antibody (1/1000 dilution). A GalNac treatment (200 ┬ÁM) was used to compete for DBA staining as specificity control. Sections were counterstained with hematoxylin. A black segment indicates the microscopy magnification scale. GC, granulosa cell layer; TC, theca cell layer; Ant, antral cavity.

Figure 4

doi: https://doi.org/10.1371/journal.pgen.1003809.g004